Early kinetic screening of hybridomas for confi dent antibody selection using Biacore A100

ثبت نشده
چکیده

Acknowledgements We gratefully acknowledge Scott Klakamp & Andy Drake (Abgenix Inc.) and David Myszka (University of Utah) for their invaluable contributions in making this collaboration possible. Figure 1. Biacore® A100 offers both rapid kinetic screening and high resolution kinetic characterization of mAbs direct from crude hybridoma samples in one versatile, high-performance system. APPLICATION NOTE 84

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Anti-peptide antibody screening: selection of high affinity monoclonal reagents by a refined surface plasmon resonance technique.

A refined surface plasmon resonance method was developed to measure the kinetics of peptide binding to rabbit monoclonal antibodies (RabMAbs). Optimized amounts of RabMAbs were captured onto sensor chips from hybridoma supernatants followed by binding of free peptides from solution. This allowed kinetic measurement of monovalent interactions of peptides with single antigen binding sites on the ...

متن کامل

t i on The Case for Quantitative Process Management

D uring development, how can you determine with confi dence whether your system will exceed its allowable downtime when delivered? When customers change their minds about functionality, how can you be confi dent that you’ve made the right trade­offs to deliver the system within your original fi xed budget? During the early weeks of a sprint, how can you be confi dent that you can deliver all th...

متن کامل

Selection of an anti-CD20, single-chain antibody by phage ELISA on fixed cells.

Cloning the correct genes that code for antibody-variable domains from hybridomas is often complicated by the presence of several immunoglobulin transcripts, some of them arising from a myeloma cell line. For the rapid functional evaluation of recombinant antibody fragments against cell-surface antigens, we established an efficient expression and screening system using phagemid antibodies and f...

متن کامل

Monoclonal Antibodies: A Tool for Molluscan Pathology

-Hybridoma technology is reviewed, and the characteristics of monoclonal antibodies are compared with those of polyclonal antibodies. The contribution of monoclonal antibodies to molluscan pathology is developed with special emphasis on their use as diagnostic tools. The results of studies with monoclonal antibodies prepared against the protozoan oyster pathogen Bonamia ostreae are briefly desc...

متن کامل

Development of an antigen microarray for high throughput monoclonal antibody selection

Monoclonal antibodies are valuable laboratory reagents and are increasingly being exploited as therapeutics to treat a range of diseases. Selecting new monoclonal antibodies that are validated to work in particular applications, despite the availability of several different techniques, can be resource intensive with uncertain outcomes. To address this, we have developed an approach that enables...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2005